rabbit anti bcan  (Novus Biologicals)

Journal: STAR Protocols

Article Title: Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

doi: 10.1016/j.xpro.2023.102681

Figure Lengend Snippet: Detailed staining conditions for each primary antibody used for mouse FFPE sections

Article Snippet: Rabbit anti-brevican (BCAN), 1:2,000 , Abcam , Cat# ab285162.

Techniques: Staining, Incubation

Journal: STAR Protocols

Article Title: Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

doi: 10.1016/j.xpro.2023.102681

Figure Lengend Snippet: Example staining strategy (A) For a single region of interest, we acquired neighboring sections with different stains, such as PLP, Neurofilament (NF), NeuN, CD68 and Ferritin close to the midline. (B) For multiple regions of interest, we acquired three different stains, PLP, NF, Brevican, per region of interest.

Article Snippet: Rabbit anti-brevican (BCAN), 1:2,000 , Abcam , Cat# ab285162.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

doi: 10.1016/j.xpro.2023.102681

Figure Lengend Snippet: MRI-histology registration chain and examples of outcomes Framework for histology-MRI registration and extraction of quantitative histology metrics. (A) Multi-modal MRI to multi-stain histology registration workflow. Pydpiper is used for the standard-space registration and atlas-based segmentation of the dMRI data. FSL-FLIRT is used to co-register the multi-modal MRI data. TIRL is used for the MRI-histology and histology-histology registration. (B) Example MRI-histology (PLP) registration, red contour line represents the white matter boundary. (C) Example Multi-modal MRI registration result. R2∗ is registered to T2-weighted MRI, and FA (Fractional anisotropy) is registered to T2-weighted MRI. (Note FA was calculated using the diffusion tensor model). (D) Example histology-histology registration result. Neurofilament (NF) is registered to the PLP section, and Brevican is registered to the PLP section for the same sample. (E) PLP slice (coronal) displayed in 3D MRI space (represented by sagittal slice). (F) Quantitative histology metrics obtained from PLP: Structure tensor imaging and Stain Area Fraction map. The color wheel represents the fiber orientation. Red contour represents the white matter tract boundary. The white matter mask was manually segmented in standard diffusion-weighted MRI space with a visual aid from an atlas-based segmentation output and a tract skeleton (G) Example of a failed registration of an overprocessed sample which has shrinkage and folds.

Article Snippet: Rabbit anti-brevican (BCAN), 1:2,000 , Abcam , Cat# ab285162.

Techniques: Extraction, Staining, Diffusion-based Assay, Imaging

Journal: STAR Protocols

Article Title: Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

doi: 10.1016/j.xpro.2023.102681

Figure Lengend Snippet: Expected outcome Representative images of Brevican (A, enlarged in B), CD68 (C, enlarged in D), Ferritin (E, enlarged in F), NeuN (G, enlarged in H), Neurofilament (I, enlarged in J) and PLP (K, enlarged in L) staining. Neurofilament (NF). Perineuronal nets (black arrowhead) and macrophages/microglia (white arrowhead). Scale bar is 50 μm. Red box indicates location of higher magnification panel.

Article Snippet: Rabbit anti-brevican (BCAN), 1:2,000 , Abcam , Cat# ab285162.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

doi: 10.1016/j.xpro.2023.102681

Figure Lengend Snippet:

Article Snippet: Rabbit anti-brevican (BCAN), 1:2,000 , Abcam , Cat# ab285162.

Techniques: Recombinant, Paraffin Wax, Injection, Saline, Electron Microscopy, Blocking Assay, Knock-Out, Mutagenesis, Software, Staining, Microscopy, Immunostaining

Journal: Nature

Article Title: Required growth facilitators propel axon regeneration across complete spinal cord injury

doi: 10.1038/s41586-018-0467-6

Figure Lengend Snippet: a, Laminin immunohistochemistry (IHC) images plus quantification (mean±SEM stained area), and dot blot plus quantification (mean±SEM density). TP, total protein. (* P <0.01, one-way ANOVA/Bonferroni). b, IHC images and quantification (mean±SEM cell number) of astrocyte proliferation and density. (ns non-significant, * P <0.0005, one-way ANOVA/Bonferroni). c, BDA-labeled axon regrowth past PB and in LC among CD13-positive stromal cells (left) and along laminin (right). White arrows denote PB. d-f, IHC images (d) and graphs of mean±SEM axon contact with laminin (e) (*** P <0.0001; Student’s two-tailed t-test, t (9)=107.4), and mean±SEM axon length per tissue volume (f) (* P <0.0005 one-way ANOVA/Bonferroni). g, CSPG dot blot (mean±SEM density). (ns non-significant, * P <0.05, one-way ANOVA/Bonferroni). For all graphs, dots show n mice per group. h, i, BDA-labeled axon regrowth through astrocytes of PB (h) and along laminin in LC (i) in spite of dense brevican (BCAN).

Article Snippet: Primary antibodies were: rabbit anti-GFAP (1:2000; Dako, Santa Clara, CA); rat anti-GFAP (1:1000, Thermofisher, Grand Island, NY); chicken anti-GFAP (1:1000, Novus Biologicals, Littleton, CO); rabbit anti NeuN (1:1000, Abcam, Cambridge, MA); rabbit anti-GDNFR-a (GDNF-receptor alpha) (1:1000, Abcam, Cambridge, MA); sheep anti-BrdU (1:300, Maine Biotechnology Services, Portland, ME); rabbit anti-HSV-TK (1:1,000, , ); goat anti-CD13 (1:1000, R&Dsystems, Minneapolis, MN); rabbit anti-Laminin 1 (1:100, Sigma, St.Louis, MO); rabbit anti-Fibronectin (1:500, Millipore, Burlington, MA); rabbit anti-Collagen 1a1 (1:300, Novus Biologicals, Littleton, CO); mouse anti-NeuN (1:2000, Millipore, Burlington, MA); mouse anti-CSPG (1:100, Sigma); rabbit anti-Brevican (BCAN) (1:300, Novus Biologicals, Littleton, CO); guinea pig anti-NG2 (CSPG4) (Drs. E.G. Hughes and D.W. Bergles , Baltimore, MA); rat anti-PECAM-1 (1:200, BD Biosciences, San Jose, CA); guinea pig anti-homer1 (1:600, Synaptic Systems GmbH, Germany); rabbit anti-Synaptophysin (1:600,Dako, Santa Clara, CA); rabbit anti-RFP (1:1000, Rockland,Limerick,PA); chicken anti-RFP (1:500, Novus Biologicals, Littleton, CO); goat anti-GFP (1:1000, Novus Biologicals, Littleton, CO).

Techniques: Immunohistochemistry, Staining, Dot Blot, Labeling, Two Tailed Test